hood rat - adult rat cardiomyocytes

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adult rat cardiomyocytes - hood rat


Ca 2+ mobilization in adult rat cardiomyocytes by angiotensin type 1 and 2 receptors. Biochem Pharmacol –, Crossref | PubMed | ISI | Google Scholar; 26 Shmigol AV, Eisner DA, and Wray S. Simultaneous measurements of changes in sarcoplasmic reticulum and cytosolic [Ca 2+] in rat uterine smooth muscle cells. J Physiol Aim: In order to get high-yield and high-quality cardiomyocytes from adult rat. We needed to clarify the optimal perfusion pressure in a constant flow condition and stopping digestion pressure. Methods: The rat was injected with heparin (1 IU/kg, i. p.) 20 minutes prior to the experimental protocol. The heart was excised and the aorta was cannulated rapidly.

In an intact heart, adjacent cells influence adult cardiomyocytes. With the method of isolation and cultivation of adult cardiomyocytes, a precise investigation of the behavior of these cells under specific treatments and environments is possible. This manuscript presents a protocol for successful isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). It has been reported that approximately 3 million cardiomyocytes could be obtained from an adult rat heart (Campora et al., ). In our protocol, about 85% cells were rod shape in the digested cells, and – × 10 6 live cardiomyocytes could be derived from one heart.

Uncoupling protein 2 modulates cell viability in adult rat cardiomyocytes. Uncoupling protein 2 (UCP2) is an inner mitochondrial membrane proton carrier that uncouples ATP synthesis. The aim of this study was to determine whether UCP2 plays a role in survival of adult rat cardiac myocytes. We first studied the effects of UCP2 overexpression in vitro. October 19th, Here, we present a protocol for the isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). Isolated ARVC can be used for short and long-term cultivation. The isolation and cultivation of ARVC can play a key role in developing new treatment regimens for cardiac diseases.